Powdered Grace’s insect culture medium

Grace successfully established the first continuous insect cell line using this medium. Grace’s insect culture medium was initially used for the cultivation of Antherea eucalypti cells in Australia. After appropriate supplementation, Grace’s supplemented insect culture medium is widely used for the growth of Sf9 and Sf21 cells of Spodoptera frugiperda. Sf9 and Sf21 cells are usually used to express recombinant proteins using the baculovirus expression vector system (BEVS). Grace’s unsupplemented insect culture medium can be used for insect cell transfection or protein labeling research (including tyrosine). Before being used as a growth culture medium, Grace’s unsupplemented insect culture medium needs to be supplemented with 10% fetal bovine serum (FBS), 3.33 g/L casein hydrolysate and 3.33 g/L yeast powder. Grace’s insect culture medium requires 5%-10% CO2 to maintain physiological pH value. For a wide range of cell culture applications, we offer various components of Grace’s modified insect culture medium, as well as customizable components of Grace’s insect culture medium.

Catalog number Specification Component description HCY-BCMP14001A 1L×10 bags Contains: L-glutamine Does not contain: casein hydrolysate, yeast powder, sodium bicarbonate HCY-BCMP14001B 10L×1 bottle HCY-BCMP14002A 1L×10 bags Contains: L-glutamine, casein hydrolysate, yeast powder Does not contain: sodium bicarbonate HCY-BCMP14002B 10L×1 bottle

Ingredients of Powdered Grace’s insect culture medium

This Grace’s dry powder cell culture medium needs to be supplemented with sodium bicarbonate to adjust pH value and filtered when prepared. The detailed preparation scheme is as follows:

1. Add 950 mL distilled water to the mixing container as close as possible to the final volume.
2. Add the dry powder culture medium to room temperature (15°C to 30°C) water and stir gently. Do not heat the water.
3. Rinse the inside of the package to remove all residual powder.
4. Add 4.7 mL of 75g/L NaHCO3 solution to the culture medium.
5. Adjust the pH value to the desired final working pH value below 0.2 to 0.3 units by slowly adding and stirring 1 N NaOH or 1 N HCl. The pH value may increase by 0.1 to 0.3 units after filtration. The recommended working pH value is 5.9-6.2.”