HCY™ Klentaq DNA Polymerase Kit

HCY™ Klentaq DNA Polymerase Kit

With a 278 amino acid deletion at the N-terminus of KlenTaq DNA Polymerase, the truncated Klentaq enzyme has higher fidelity and thermal stability compared to wild-type Taq enzymes. The enzyme retained the DNA polymerase activity of DNA polymerase I and 3’→5′ nucleic acid exonuclease activity, and lacked 5’→3′ nucleic acid exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.

Source

HCY™ KlenTaq DNA Polymerase is Recombinant KlenTaq DNA Polymerase, which is genetically modified by mutation and recombination, and is obtained by expression purification in E. coli.

Uses

• Conventional PCR, high-fidelity PCR, genotyping and primer extension analysis, etc.
• HCY™ KlenTaq enzyme can amplify DNA fragments up to 5 kb in length. It is usually suitable for amplification of DNA fragments up to 3 kb in length.
• Most PCR products amplified with this product have an “A” base at the 3′ end and can therefore be cloned directly into the T-Vector.

Activation Definition

Using activated salmon sperm DNA as template/primer, the activity of 10 nmol of whole nucleotides ingested as acid insoluble was defined as 1 unit of activity (U) at 74°C for 30 min.

Performance of HCY™ Klentaq DNA Polymerase

HCY™ KlenTaq DNA Polymerase Amplified Fragment Lengths

• KlenTaq DNA Polymerase amplifies 0.5kb/1kb/2kb/5kb fragments, which shows that KlenTaq DNA Polymerase can efficiently amplify λDNA fragments of different lengths (0-5 kb).
• M indicates DL10,00 DNA Marker.

HCY™ KlenTaq DNA Polymerase Amplification Sensitivity

• Amplification of E. coli 300bp fragment with the addition of 0pg/0.01pg/0.1pg/1pg/10pg/100pg/1ng/10ng/100ng concentration of templates; it is evident that this product can amplify E. coli genes with template amounts as low as 100pg.
• NC indicates KlenTaq DNA Polymerase amplification without template negative control.

HCY™ KlenTaq DNA Polymerase Amplification Speed

• The extension time was 30 sec./60 sec./120 sec. for amplification of 0.5kb/1kb/2kb/5kb size target fragments;
• It can be seen that the extension time of 30 sec. and 60 sec. can well amplify 2kb and 5kb DNA fragments, respectively, indicating that the PCR reaction system with λ DNA as template can set the extension time according to 5kb/min.
• M is for DL10,000 DNA Marker.

Quality Control

• SDS-PAGE electrophoretic purity greater than 98%;
• Functional test: sensitivity, specificity, reproducibility of PCR;
• No exogenous nuclease activity, no exogenous endonuclease, and exonuclease contamination;
• No expression of host nucleic acid residue.

Precautions: This product is for scientific research only and cannot be used for medical or diagnostic procedures in humans or animals or as food, cosmetics or household products. Without written permission, authorization, or approval, this product may not be manufactured, licensed for sale, sold, imported or exported, or use all related patents and trademarks of the product.

Packing List

Storage Conditions

For long term storage, please store at -20℃ away from light. If frequent use is required, it can be divided and stored at 4℃ to avoid repeated freezing and thawing. After thawing, it should be mixed well to avoid generating a lot of air bubbles.