HCY™ HS-Taq DNA Polymerase Kit

HCY™ HS-Taq DNA Polymerase Kit

HCY™ HS-Taq DNA Polymerase activity is activated only after 10 minutes of pre-denaturation at 98°C, which inhibits non-specific amplification caused by non-specific annealing of the primers or primer dimerization at low temperatures. HCY™ HS-Taq DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity, no 3′-5′ exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.

Source

HCY™ Taq DNA Polymerase is Recombinant Taq DNA Polymerases obtained by E. coli expression purification and modified by a special chemical modification method.

Uses

• PCR with a high background and with non-specific bands.
• Reaction systems can be formulated at room temperature.

Activation Definition

Using activated salmon sperm DNA as template/primer, the activity of 10 nmol of whole nucleotides ingested as acid insoluble was defined as 1 unit of activity (U) at 74°C for 30 min.

Performance of HCY™ Pfu DNA Polymerase

HCY™ HS-Taq DNA Polymeras hot start performance validation:

• HCY™ HS-Taq DNA polymerase activity is activated only after a 10-minute pre-denaturation at 98°C, which inhibits non-specific amplification under room temperature conditions.
• M indicates DL10,000 DNA Marker.

Comparison of amplification results between HCY™ HS-Taq DNA Polymeras and other manufacturers’ taq enzymes in room temperature configured reaction systems:

• At room temperature, the reaction system was formulated with no primer dimer generation for non-specific amplification, whereas the comparison shows dimer formation in the amplification of other manufacturers’ enzyme solutions under the same conditions.
• M indicates DL2000 DNA Marker.

Quality Control

• SDS-PAGE electrophoretic purity greater than 98%;
• Functional test: sensitivity, specificity, reproducibility of PCR;
• No exogenous nuclease activity, no exogenous endonuclease, and exonuclease contamination;
• No expression of host nucleic acid residue.

Precautions: This product is for scientific research only and cannot be used for medical or diagnostic procedures in humans or animals or as food, cosmetics or household products. Without written permission, authorization, or approval, this product may not be manufactured, licensed for sale, sold, imported or exported, or use all related patents and trademarks of the product.

Packing List

Storage Conditions

For long term storage, please store at -20℃ away from light. If frequent use is required, it can be divided and stored at 4℃ to avoid repeated freezing and thawing. After thawing, it should be mixed well to avoid generating a lot of air bubbles.