Fischer’s culture medium was designed by Glen Fischer at the National Cancer Institute and was originally used for the culture of murine leukemia cells.
Now Fischer’s Culture Medium is mainly used in the culture of hematopoietic cells, such as lymphoblastoid and lymphocyte culture.
Ingredients of Fischer’s Culture Medium
Cat No. HCY-BCM15004
Form
Fluid
Concentration
1×
Specification
500mL
PH
7.2~7.4
L-Glutamine
204mg/L
NaHCO3
1125mg/L
D-glucose
1000mg/L
Sodium Pyruvate
FREE
HEPES
FREE
PSP
5mg/L
Storage Condition
2℃~8℃,Keep in dark place
Transportation Condition
Room temperature
Expiration Date
12 months
L-glutamine (Glutamine)
L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will degrade spontaneously. The amount of L-glutamine can be arbitrarily adjusted according to the research needs of the medium without L-glutamine, and the addition of fresh L-glutamine or its substitutes at the time of use is more beneficial to cell growth.
L-alanyl-L-glutamine (Ala-Glu), also known as alanyl-glutamine and alanyl-glutamyl dipeptide, is an advanced cell culture additive that can directly replace L-glutamine in the cell culture medium. l-Glutamine is an essential nutrient in cell culture, but it is unstable in solution and will, however, it is unstable in solution and spontaneously degrades to produce ammonia and pyroglutamic acid, of which ammonia is harmful to cells, whereas L-alanyl-L-glutamine is very stable in aqueous solution and does not degrade spontaneously. The mechanism of cellular utilization is that during cell culture, cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, and then cells will take up and utilize these two hydrolysis products. The process of cellular utilization of L-alanyl-L-glutamyl is similar to the flow-addition culture strategy, in that low levels of L-glutamine are continuously added to the culture medium, thereby increasing the utilization of L-glutamine without generating excess ammonia, which is more conducive to cell growth. L-alanyl-L-glutamyl can replace equimolar L-glutamine, is suitable for all cells, requires little adaptation, and can extend cell culture time. It can also extend the cell culture time and reduce the number of passages, which saves time and money. Cells cultured in medium supplemented with L-glutamine showed a slower reduction in activity than those cultured in a medium supplemented with L-glutamine. The slightly longer delay period is due to the time required for the release of peptidase and the digestion of the dipeptide.
Cautions of McCoy’s 5A Medium
This product has been filtered and de-bacterized and should be used with care for the aseptic operation to avoid contamination.
Do not freeze-thaw the product in order to maintain the best use of the product.
This product is intended for scientific research or further study use only, not for diagnosis or treatment.