Feline Panleukopenia Virus Antibody Test Kit (FPV-Ab): colloidal gold

Test principle of the Feline Panleukopenia Virus Antibody Test Kit

Test principle of the Feline Panleukopenia Virus Antibody Test Kit uses a rapid immunochromatographic detection technique for the detection of antibodies to feline fever virus. A sample obtained from cat whole blood, serum or plasma is added to the spiked wells and moved along the chromatographic membrane together with colloidal gold-labeled FPV antigen. If FPV antibodies are present in the sample, they bind to the antigen on the detection line (T) and show a burgundy color. If FPV antibodies are not present in the sample, no color reaction is produced.

The Feline Panleukopenia Virus Test Kit is able to detect Feline Panleukopenia Virus antibody concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Feline Panleukopenia Virus in feline whole blood, serum, or plasma. (FPV).

Components of the FPV-Ab Test Kit

Storage conditions and Validity Period

Store at 2-30°C, valid for 24 months.

Sample Requirements

• The Feline Panleukopenia Virus Antibody Test Kit is only suitable for testing cat whole blood, serum or plasma samples.
• Fresh samples are collected for testing and should be tested as soon as possible within 1 hour.
• If the test cannot be performed in time, whole blood, serum or plasma samples can be stored at 2-8°C for 3 days and serum or plasma samples can be stored at -20°C for 1 month.

How to use Feline Panleukopenia Virus Antibody Test Kit

1. Collect a sample of cat whole blood, serum or plasma, add the sample to the diluent after collection, tighten the cap, and mix upside down.
2. Tear open the foil pouch of the test card, remove the test card and place it on a flat, clean benchtop.
3. Pipette 3 drops (about 80 μl) of the diluted sample into the spiked well of the test card and wait for the test results.
4. Wait for 10-15 min at room temperature to read the test results, after 20 min the test results are invalid.

Note: The above reading time is at room temperature 15-30 ℃, if the temperature is lower than 15 ℃, the reading time should be extended appropriately. If the temperature is higher than 30 ℃, should be appropriate to shorten the reading time.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Feline Panleukopenia Virus Antibody Test Kit

• Please read the instructions carefully before the test. The various reagents provided with this product are for this test only.
• Products with expired or broken aluminum foil bags should not be used.
• If the kit is taken out under low temperature storage, it should be opened after restoring to room temperature, and the opened test card should be used as soon as possible to avoid moisture, and should not be used for more than 1 hour.
• Avoid contact with the sample filling holes on the test card and the chromatographic strips at the test window.
• The waste after the test should be considered as a contaminant and properly disposed of according to the relevant local regulations.