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Feline herpesvirus PCR Detection KitFeline Herpesvirus (FHV-1) Real-time PCR Detection Kit (Lyophilized Bead)
Feline herpesvirus PCR Detection KitFeline Herpesvirus (FHV-1) Real-time PCR Detection Kit (Lyophilized Bead)
Feline herpesvirus PCR Detection KitFeline Herpesvirus (FHV-1) Real-time PCR Detection Kit (Lyophilized Bead)
PCR Detection KitFeline Herpesvirus (FHV-1) Real-time PCR Detection Kit (Lyophilized Bead)

Feline Herpesvirus (FHV-1) Real-time PCR Detection Kit (Lyophilized Bead)

Cat. No.: CY-FHV-LM10

HCY™ Feline Herpesvirus (FHV-1) Fluorescent Quantitative Test Kit (Lyophilized Microspheres) is a fluorescent quantitative assay for the detection of feline herpesvirus in samples of nasopharyngeal swabs, conjunctival secretions, blood and organs. Specific primers and Taqman probe are designed with the conserved gene of FHV-1, and amplified by fluorescence quantitative PCR instrument. Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of the whole process of indoor quality control from sampling, sample purification to on-line testing.

PRODUCT DETAIL
INQUIRY

HCY™ Feline herpesvirus Real-time PCR Detection Kit

  • Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
  • The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
  • Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
  • Easy to use, no need to prepare reagents in advance;
  • The minimum detection limit is 2 copies/Test;
  • Clinical sensitivity 100%, specificity 100%;
  • Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

An evolutionary tree was constructed based on the sequences of FHV-1 isolates. The sequences of virulent strain YBYJ1-33HR, virulent strains, and other Chinese feline herpes sequences indexed on NCBI in China were compared with the sequences of NCBI reference strains (NC_013590.2), and the sequences of other countries’ regions FHV_CU1-112 (Thailand), MH070323-MH070348, ( USA) and other sequences were compared for homology. An evolutionary tree was constructed to analyze the homology between 99.2-99.5%.

HCY™ Feline herpesvirus Real-time PCR Detection Kit is designed and developed based on the gene sequences of endemic pathogens in different regions of the world, as mentioned above, which fully ensures the accuracy and validity of the detection. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 2 copies/Test. Calculation of the experimental data set yielded R2=0.995 and the amplification efficiency E=98.71%.

Feline herpesvirus PCR Detection Kit
Amplification Curve
Feline herpesvirus PCR Detection Kit
Amplification Efficiency

Sensitivity and Specificity

The HCY™ Feline herpesvirus Real-time PCR Detection Kit was compared to similar commercially available products and tested a total of 127 real clinical samples, of which the sensitivity was 100% and the specificity 100%.

Reference ProductTotal
NegativePositive
HCY™ PCR Test KitPositive01414
Negative1130113
Total11314127

Estimated Value95% Confidence Interval
Sensitivity100%73.24%~100%
Specificity100%95.90%~100%

Cross-reactivity

The HCY™ Feline herpesvirus Real-time PCR Detection Kit has been validated for the detection of Bartonella, Borrelia burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica Feline Calicivirus, Feline coronavirus, Feline Parvovirus, Chlamydia felis, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma haemominutum. Candidatus Mycoplasma turicensis, Canine parainfluenza Virus, Canine Coronavirus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus. None of them cross-reacted.

“HCY, Health care for you” is our forever mission. We dedicate to offering safe & reliable products and medical services with our global creditable partners. HCY has already supplied to WHO, MAYO clinic, MGI, DDC, Yale University, Qorvo, Quanterix, Thomas Scientific, SD biosensor, Cardinal Health, Cleveland Clinic, Mars Petcare & LumiraDx, etc. in the past years.

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