2019-nCoV Ag Rapid Test Kit: Lollipop Rapid Saliva Test

The reagent membrane on the 2019-nCoV ag lollipop rapid test kit contains the colloidal-gold conjugated with the monoclonal antibodies against Novel coronavirus. The reaction membrane contains the secondary antibodies for Novel coronavirus, and the polyclonal antibodies against the mouse globulin, which are preimmobolized on the membrane.

Guide 1 for 2019-nCoV lollipop rapid saliva test

1. After washing your hands, take the test kit out of the aluminum foil bag and remove the blue lid of the test lollipop.
2. Lower the tip of the tongue and the upper jaw of the mouth and wait for the saliva to fill the mouth, put the absorbent tip of the best lollipop into the mouth (all the absorbent tip is pressed under the tongue), and place the absorbent tip beneath the tongue for 90 seconds.
3. Take out the test lollipop from the mouth and lid the blue of the test lollipop. Place the test lollipop horizontally, read the displayed result within 15 minutes, and the results read after 30 minutes are invalid.
4. Safely throw away the waste into a biohazard container.

Guide 2 for COVID-19 lollipop rapid saliva test kit

1. Spit saliva to the saliva collector.
2. Take out the test lollipop, put the saliva adsorption stick into the saliva collector, start the timer. After about 90 seconds, cover the lid of the saliva adsorption stick.
3. Pace the detection lollipop horizontally on the workbench, and read the detection result 15 minutes later.The test result should not be read and interpreted after 30 minutes.
4. Safely throw away the waste into biohazard containers.

Interpretation of Results

This kit collects saliva samples from the saliva adsorption stick at the front of the test card, if the sample contains a SARS-CoV-2 N protein antigen, it is identified and bound by antibodies labeled on the surface to form an immune complex.

Positive:

Two colored lines appear. One red-colored line next to “C” and one red-colored line next to “T” indicates SARSCoV-2 positive result.

Negative:

One red-colored line only next to “C” indicates a negative result.

Invalid:

If the red-colored line in the control region “C” is not visible, the result is invalid.

Precautions for using lollipop rapid saliva test kit

1. For in vitro diagnostic use only.
2. Allow the test device to equilibrate to room temperature (25℃-30℃) prior to testing.
3. Do not eat, drink, or smoke for at least 30 minutes before the test.
4. Do not use it after the expiration date.
5. Ensurefoilpouchcontaining test device is not damaged before opening for use.
6. Perform the test at room temperature from 15℃ to 30℃.
7. Wear gloves when hanging the samples, and avoid touching the reagent, embrace and sample window.
8. All samples and used accessories should be treated as infectious and discarded according to local regulations.
9. Avoid using blood samples.
10. Antigen detection cannot be used alone to diagnose SARS-CoV-2 infection, and the infection status should be determined in combination with nucleic acid detection, imaging, and other diagnostic information, as well as medical history and contact history.